VentDNA Polymerase from New England Biolabs

Product Specs
ItemVentDNA Polymerase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0254L
Formatunits/ml
Quantity1000 units
Concentration2,000 units/ml
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Comments:
Item name is changed

Buffer:
1X ThermoPol Reaction Buffer:

20 mM Tris-HCl

10 mM (NH₄)₂SO₄

10 mM KCl

2 mM MgSO₄

0.1 % Triton X-100

pH 8.8 @ 25°C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A conditional RNA Pol II mono-promoter drives HIV-inducible, CRISPR-mediated cyclin T1 suppression and HIV inhibitionMolecular Therapy - Nucleic AcidsJune 13, 2023Srinivasan Chinnapaiyan, Maria-Jose Santiago, Kingshuk Panda, Md. Sohanur Rahman, Jessica Alluin, John Rossi, Hoshang J. Unwallaone nucleotide. All PCR amplifications were performed using the high-fidelity Vent polymerase (NEB #M0254S). Cell culture experiments HeLa-CD4 and HIV-infected HeLa-CD4 cells were obtained from NIH AIDS Reagent

(2) The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transitionMolecular cellSeptember 15, 2022Zonderland G, Vanzo R, Gadi SA, Martín-Doncel E, Coscia F, Mund A, Lerdrup M, Benada J, Boos D, Toledo L.4-88,Sigma-Aldrich,Cat# 81381 Thymidine,Sigma-Aldrich,Cat# T1895 Vent® DNA Polymerase,New England Biolabs,Cat# M0254S XL413 (CDC7 inhibitor),AdooQ Bioscience,Cat# A21220 ,, Deposited Data,Deposited Data,Deposited Data ,, Raw

(3) The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter PaperJournal of Tropical MedicineAugust 13, 2022Zhi-yong Tao et al.Plus,B500013-0100,3.45,20,0.17,0.00,0,0.00 StarLighter Blood Direct PCR,FS-P6001,18.83,500,0.04,0.00,0,0.00 NEB Vent,M0254 V,61.03,200,0.31,0.00,0,0.00 Cost-effectiveness comparison of 20 DNA polymerases. ∗ Scores were

(4) Gene-Specific MicroRNA Antagonism Protects Against HIV Tat And TGF-β-mediated Suppression of CFTR mRNA and function.Biomedicine & PharmacotherapyOctober 1, 2021R.K. Dutta, S. Chinnapaiyan, M.J. Santiago, I. Rahman, H.J. Unwallaamplified using primer pairs flanking the 1500 bp CFTR UTR with the high-fidelity Vent polymerase (NEB #M0254S). The PCR product was then sequenced using a 5’ primer designed to hybridize just upstream of the CFTR

(5) Mechanical Tension Promotes Formation of Gastrulation-like Nodes and Patterns Mesoderm Specification in Human Embryonic Stem CellsDevelopmental CellDecember 1, 2020Jonathon M. Muncie, Nadia M.E. Ayad, Johnathon N. Lakins, Xufeng Xue, Jianping Fu, Valerie M. Weaver161–0800 Tth DNA Polymerase,Sigma,Cat# 11480022001 Vent DNA Polymerase,"New England BioLabs, Inc.",Cat# M0254S Y-27632 dihydrochloride (ROCK inhibitor),Tocris,Cat# 1254 Critical Commercial Assays,Critical Commercial

(6) New Insight into the Mechanism of Anaerobic Heme DegradationBiochemistryNovember 19, 2019Liju G. Mathew, Nathaniel R. Beattie, Clayton Pritchett, William N. Lanzilottapreheating each condition at 95 °C for 5 min, 1.0 μL of VentR DNA Polymerase (New England Biolabs; #M0254S) was added to each tube, and the PCR reactions were allowed to proceed (annealing: 55–58 °C, 1 min;

(7) Cut-and-Run: A Distinct Mechanism by which V(D)J Recombination Causes Genome InstabilityMolecular CellMay 2, 2019Christopher M. Kirkham, James N.F. Scott, Xiaoling Wang, Alastair L. Smith, Adam P. Kupinski, Anthony M. Ford, David R. Westhead, Peter G. Stockley, Roman Tuma, Joan BoyesK,Roche,Cat#03115879001 NheI,NEB,Cat#R3131 ApaI,NEB,Cat#R0114 EcoRV,NEB,Cat#R0195 ApaLI,NEB,Cat#R0507 Klenow DNA polymerase,NEB,Cat#M0212 T4 DNA ligase,NEB,Cat#M0202 Vent (exo−) DNA polymerase,NEB,Cat#M0254 Taq DNA polymerase,NEB,Cat#M0267 DNase I,Worthington,Cat#DPRF α-32P dCTP,Perkin Elmer,Cat#NEG013H γ-32P

(8) Human serum antibodies against EBV latent membrane protein 1 cross-react with α-synucleinNeurology - Neuroimmunology NeuroinflammationAugust 1, 2016John Woulfe, Madison T. Gray, Munisha S. Ganesh, Jaap M. Middeldorpwild-type a-syn-GFP was amplified in the presence of either primer pair using Vent DNA polymerase (M0254L; New England Biolabs, Ipswich, MA) to produce a blunt-ended product. Resultant PCR products were digested

(9) Analyzing the Role of Periplasmic Folding Factors in the Biogenesis of OMPs and Members of the Type V Secretion SystemMethods in Molecular BiologyOctober 3, 2015Gustavo Bodelón, Elvira Marín, Luis Ángel Fernández(Bio-Rad, 1652086). 4. Agarose. Use at 0.75–1.5% for analysis of PCR products. 5. Vent DNA polymerase (NEB, M0254S). Enzyme used for generating PCR recombineering substrates. 6. Taq DNA polymerase. Enzyme used for colony PCR

(10) Reconstituting ParA/ParB-mediated transport of DNA cargoMethods in Cell BiologyMay 23, 2015Anthony G. Vecchiarelli, James A. Taylor, Kiyoshi Mizuuchireagents, and equipment 1.3.2.1.1 Buffers ThermoPol Reaction Buffer (New England Biolabs, Cat. # M0254S) Wash Buffer: 10 mM Tris–HCl (pH 8.2), 1 M NaCl, 1 mM EDTA Binding Buffer: 10 mM Tris–HCl (pH 8.2),

(11) Spectroscopic evidence for a 5-coordinate oxygenic ligated high spin ferric heme moiety in the Neisseria meningitidis hemoglobin binding receptorBiochimica et Biophysica Acta (BBA) - General SubjectsOctober 1, 2014David Z. Mokry, Angela Nadia-Albete, Michael K. Johnson, Gudrun S. Lukat-Rodgers, Kenton R. Rodgers, William N. Lanzilottapreheating each condition at 95 °C for 5 min, 1.0 μL of VentR® DNA Polymerase (New England Biolabs; #M0254S) was added to each tube, and the PCRs were allowed to proceed (annealing: 55–58 °C, 1 min; extension:

(12) Structure of 5-hydroxymethylcytosine-specific restriction enzyme, AbaSI, in complex with DNANucleic Acids ResearchJuly 8, 2014John R. Horton, Janine G. Borgaro, Rose M. Griggs, Aine Quimby, Shengxi Guan, Xing Zhang, Geoffrey G. Wilson, Yu Zheng, Zhenyu Zhu, Xiaodong Chengsubstitution mutagenesis was carried out by polymerase chain reaction (PCR) using vent polymerase (NEB #M0254) and pairs of synthetic mutagenic oligos (IDT). The PCR products were digested with DpnI to reduce template

(13) Structure and cleavage activity of the tetrameric MspJI DNA modification-dependent restriction endonucleaseNucleic Acids ResearchOctober 1, 2012John R. Horton, Megumu Yamada Mabuchi, Devora Cohen-Karni, Xing Zhang, Rose M. Griggs, Mala Samaranayake, Richard J. Roberts, Yu Zheng, Xiaodong Chengmutation sequences. Each 50 µl inverse PCR reaction contained 2 U of the VentR DNA Polymerase (NEB #M0254), 1× ThermoPol Reaction Buffer, 200 μM dNTP Solution Mix (NEB #N0446), 0.9 µM forward and reverse primer,

(14) Prevention of Transcriptional Silencing by a Replicator-Binding Complex Consisting of SWI/SNF, MeCP1, and hnRNP C1/C2Molecular and Cellular BiologyAugust 15, 2011Liang Huang, Haiqing Fu, Chii Mei Lin, Amy L. Conner, Ya Zhang, Mirit I. Aladjem(5⬘-ACTCC TAAGCCAGTGCCAGA-3⬘) and incubating the sample at 57°C with 1 U of Vent exopolymerase (catalog no. M0254L; New England BioLabs) to obtain blunt-end products. For blunt-end ligation, we added 30 ␮l of linker

(15) Multigene Evidence for the Placement of a Heterotrophic Amoeboid Lineage Leukarachnion sp. among Photosynthetic StramenopilesProtistJanuary 3, 2009Jessica Grant, Yonas I. Tekle, O. Roger Anderson, David J. Patterson, Laura A. Katz

(16) Polymerase Amplification, Cloning, and Gene Expression of Benzo-Homologous “yDNA” Base PairsChemBioChemDecember 15, 2008Jijumon Chelliserrykattil, Haige Lu, Alex H. F. Lee, Eric T. Koolwith reaction buffer and Vent exo− polymerase (both from New England Biolabs, Ipswich, MA; Cat No: M0254S) and adjusted to a final reaction volume of 50 µL with water. The reaction conditions consisted of an

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